3D Clear Tissue Clearing Reagents  Corning®
3D Clear Tissue Clearing Reagents
Corning®


The improved in vivo relevancy of 3D cell culture has driven the adoption of these models in drug discovery. However, the imaging techniques used to characterise these models are highly limited.

Due to the thickness and opacity of these 3D cellular structures, current imaging technologies cannot penetrate to the center of the tissues, resulting in only the outer 2 to 3 layers of cells being detected. This causes the dark centers often seen in images of 3D cell culture models, which proves highly problematic for accurate analysis as these outer cells are most exposed to compounds, nutrients and oxygen, and thus do not reflect the entire cell population.

Corning 3D Clear tissue clearing reagent can be used as a tissue clearing technique designed specifically to support imaging for 3D cell culture models and plate-based high throughput processing.

When paired with fluorescent labeling (e. g., fluorescent protein, immunofluorescence, chemical dyes) and high content confocal microscopy, it allows for complete 3D cell culture model characterisation and more accurate drug screening.

Why use Corning 3D Clear Tissue Clearing Reagent?
While there are dozens of tissue clearing techniques described in literature, there are few that have been demonstrated for use with 3D cell culture models. Additionally, most of these tissue clearing techniques are not designed for compatibility with microplates (e. g., iDISCO, BABB, uDISCO, 3DISCO) or high throughput processing (e. g., CLARITY) and many are not compatible with immunofluorescent labeling (ScaleS4, CUBIC) or have high toxicity (e. g., ClearT).

Therefore, the 3D clear tissue clearing technique was designed to be a rapid, easy-to-use process that is compatible with microplates imaging, fluorescent protein and immunofluorescence.
  • Rapid tissue clearing.
  • Easy-to-use.
  • No special equipment required.
  • Compatible with immunofluorescent (IF), fluorescent protein (FP) and other fluorescent labels.
  • Microplate and automation compatible.
  • Reversible for follow up 2D H&E / IHC.

Items supplied:
Corning® 3D clear reagent (30 ml), 3D clear antibody buffer (30 ml), 3D clear blocking buffer (30 ml), 3D clear penetration buffer (30 ml), 3D clear washing buffer 10 x (70 ml).
DescriptionOrder No.PU Manuf. Prod. No. 
3D starter kitCORN57301-5730